Author

Feifei Bao

Abstract

The p53 tumor suppressor protein is involved in multiple central cellular processes and human cancer occurrences. A research effort is proposed to collect the majority of p53 ChIP fragments up to now and reveal the binding pattern of p53 binding sites. It demonstrates that the normal and cancer cell lines have significantly different chromatin organizations around P53 binding sites. Based on the high occurrences, the core binding sites can be collected to analyze gene expression configurations. Depending on the accessibility of p53 sites and epigenetic marks in the chromatin context, p53 binds to its target sites in repetitive regions. Finally, the functional annotation analysis illustrates that the most enriched pathway of p53 ChIP fragments is p53 signaling pathway and highly enriched clusters relating to apoptosis, DNA damage and cellular signaling are modulated by p53.

Library of Congress Subject Headings

p53 protein--Analysis; Chromatin

Publication Date

12-1-2016

Document Type

Thesis

Student Type

Graduate

Degree Name

Bioinformatics (MS)

Department, Program, or Center

Thomas H. Gosnell School of Life Sciences (COS)

Advisor

Feng Cui

Advisor/Committee Member

Gary R. Skuse

Advisor/Committee Member

Gregory Babbitt

Comments

Physical copy available from RIT's Wallace Library at QP552.P25 B36 2016

Campus

RIT – Main Campus

Plan Codes

BIOINFO-MS

COinS