The goal of this study was to identify BPD markers by gene expression profiling in lung and peripheral blood mononuclear cell (PBMC) gene expression data. Studies of BPD are often focused on lung tissue, but rarely venture to find markers of the disease outside of the most affected tissue. Genes identified from these analyses can be used as markers of BPD, and may also be linked with causal mechanisms of the disease. First, we tested the biological relevance of BPD biomarkers previously identified in lung tissue by assessing their importance in normal lung development. Next, we identified sets of differentially expressed genes at 5±2 days (Near-birth) and 25±3 days (Near-diagnosis) post birth within a publicly-available microarray dataset utilizing RNA from the PBMCs from infants at high risk for a diagnosis of BPD. These lists provided markers of BPD including ARG1, MPO, OLFM4, CEACAM1, CEACAM6 and CEACAM8 that may provide further insight into the physiology of infants with BPD. Of these, ARG1, CEACAM1 and CEACAM6 were validated by qPCR using RNA samples from lung tissue. The identified genes and their associations with the inflammatory response and vascular development via the Urea cycle provide a basis for further investigation of these pathways within BPD pathogenesis. Another benefit of these markers has been explored: using PBMC gene expression to predict long-term outcome in the near-birth time point. These studies showed that a simple 3-fold cross-validation technique could provide a model with nearly 73% accurate classification of infants at less than 1 week after birth. These studies provide a strong motive for further analyses of these genes of interest and the predictive ability of biomarkers of BPD in PBMCs.
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Department, Program, or Center
Thomas H. Gosnell School of Life Sciences (COS)
Mereness, Jared A., "Genes of Interest in Bronchopulmonary Dysplasia: Studies of Genome-Wide Expression Profiling Data from Lung and Peripheral Blood Samples" (2013). Thesis. Rochester Institute of Technology. Accessed from
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