Multiple transposable elements have been identified by colocalization analysis that display a strong predicted regulatory relationship with p53 associated peaks. RNA-Seq was used to identify differentially expressed transposable elements. ChIP-Seq was used to identify peaks representing transcription factor binding sites in p53 activated cells. The results of both experiments were then combined in a colocalization analysis identifying transposable element locations that were both differentially regulated and located near p53 associated peaks. The colocalization of ChIP-Seq and RNA-Seq analyses allows for the verification of p53’s regulatory role in the expression of transposable elements across the genome. A Monte Carlo simulation was performed verifying that the frequency of the colocalizations observed occurred more frequently than due to random chance.
Library of Congress Subject Headings
Genetic regulation; Tumor suppressor proteins; Proteomics; Transposons
Department, Program, or Center
Thomas H. Gosnell School of Life Sciences (COS)
Leslie Kate Wright
Rosato, Andrew J., "Regulation of Transposable Elements by Tumor Suppressor Protein 53" (2020). Thesis. Rochester Institute of Technology. Accessed from
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