Abstract
Multiple transposable elements have been identified by colocalization analysis that display a strong predicted regulatory relationship with p53 associated peaks. RNA-Seq was used to identify differentially expressed transposable elements. ChIP-Seq was used to identify peaks representing transcription factor binding sites in p53 activated cells. The results of both experiments were then combined in a colocalization analysis identifying transposable element locations that were both differentially regulated and located near p53 associated peaks. The colocalization of ChIP-Seq and RNA-Seq analyses allows for the verification of p53’s regulatory role in the expression of transposable elements across the genome. A Monte Carlo simulation was performed verifying that the frequency of the colocalizations observed occurred more frequently than due to random chance.
Library of Congress Subject Headings
Genetic regulation; Tumor suppressor proteins; Proteomics; Transposons
Publication Date
12-3-2020
Document Type
Thesis
Student Type
Graduate
Degree Name
Bioinformatics (MS)
Department, Program, or Center
Thomas H. Gosnell School of Life Sciences (COS)
Advisor
Gregory Babbitt
Advisor/Committee Member
Feng Cui
Advisor/Committee Member
Leslie Kate Wright
Recommended Citation
Rosato, Andrew J., "Regulation of Transposable Elements by Tumor Suppressor Protein 53" (2020). Thesis. Rochester Institute of Technology. Accessed from
https://repository.rit.edu/theses/10626
Supplement
Campus
RIT – Main Campus
Plan Codes
BIOINFO-MS
