The focus of this thesis project was to investigate the impact of cold temperature conditions on the resistance of Vitis vinifera (grapevines) to powdery mildew, a phenomenon known as cold stress-induced disease resistance (SIDR). The model organism Arabidopsis thaliana was used to determine specific defense mechanisms of plant-pathogen resistance. An RNA-Seq time course experiment was performed for the two organisms: V. vinifera and A. thaliana. The time-series datasets consisted of data points where samples were exposed to an acute (less than 4 hours) cold (below 8°C) temperature for set times prior to inoculation with powdery mildew. The acute cold time points used ranged from 48 hours, 36 hours, 24 hours, and 12 hours prior to inoculation. An untreated control group, which was not exposed to any acute cold treatment, was used to compare between the treatments. The significant, differentially expressed genes were evaluated and mapped to the pathways of the respective organisms. The outcome of this project was the identification of pathways, as well as potential genes of interest, involved with cold stress-induced disease resistance. There has been limited research on genetic mechanisms of cold stress induced disease resistance. This project provides an improved understanding of the interactions between host stress and the epidemiology of the biotrophic pathogen powdery mildew.
Department, Program, or Center
Thomas H. Gosnell School of Life Sciences (COS)
Michael V. Osier
Palumbo, Cal D., "Investigation of Cold Stress Induced Disease Resistance (SIDR) in Grapevines and Arabidopsis" (2019). Thesis. Rochester Institute of Technology. Accessed from
RIT – Main Campus
Available for download on Tuesday, November 05, 2019