Greg Wright

Abstract

The positions of nucleosomes were studied to determine the relationship between sequence and relative position to transcription start sites. Five sets of nucleosome data were analyzed from yeast and human cell lines; the nucleosomes were categorized based on the weak and strong dinucleotide frequency within the major and minor grooves of the nucleosomal DNA. The relative positions and density of nucleosomes were determined around transcription start sites and compared to gene expression data. The yeast data sets consisted of different collection techniques and cell environments to confirm that the nucleosome tendencies were not a factor of a particular data set. The yeast data was compared to the human data set to find similarities and differences of the observed tendencies. What follows is a comparison between the different data sets as a whole and grouped by gene expressions.

Library of Congress Subject Headings

Eukaryotic cells--Genetics; Nucleoproteins; Genetic transcription; Yeast fungi--Genetics; Human genome

Publication Date

8-19-2014

Document Type

Thesis

Student Type

Graduate

Degree Name

Bioinformatics (MS)

Department, Program, or Center

Thomas H. Gosnell School of Life Sciences (COS)

Advisor

Feng Cui

Advisor/Committee Member

Michael V. Osier

Advisor/Committee Member

Gary R. Skuse

Comments

Physical copy available from RIT's Wallace Library at QP625.N87 W74 2014

Campus

RIT – Main Campus

Plan Codes

BIOINFO-MS

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