Researchers have extensively studied the techniques and applications of Particle Image Velocimetry (PIV) and Planar Laser Induced Fluorescence (PLIF). Developments in computing and imaging techniques have significantly aided the accuracy and efficiency techniques which are now commonly used to study velocity vectors, predicting acceleration and mixing profiles in modern fluid dynamics. This project focused on analyzing the flow field inside a MicroWell, a component of the assay kits that are processed by the Vitros ECi analyzer. The assay kits and analyzer are manufactured by Ortho Clinical Diagnostics, Rochester, NY. Reagents and samples are mixed in the MicroWell during various operations of the analyzer. This process was extensively examined and quantified using the said techniques. This study involved building a test rig that emulates the injection and mixing process of the actual system and allows access for extensive PIV and PLIF analysis as well as optimization and synchronization of both systems for accurate real-time results. Further, results obtained from both experiments were studied in conjunction and then quantified for an aid of future assay and Immunodiagnostics development.
Library of Congress Subject Headings
Medical instruments and apparatus--Testing; Fluid dynamic measurements; Particle image velocimetry; Fluorescence; Biomedical engineering
Department, Program, or Center
Mechanical Engineering (KGCOE)
Chainani, Amit, "Application of non-invasive flow measurement techniques for quantitative analysis of a biomedical device" (2011). Thesis. Rochester Institute of Technology. Accessed from
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