The effects of the enzyme papain on triarylmethane photochromism were investigated. The interaction between papain solutions and triarylmethane dyes was measured using difference spectroscopy and relative equilibrium constants were calculated. The effects of papain on Malachite Green photochromic solutions were also measured. Both crude and commercially available purified papain were tested. Crude papain bleached triarylmethane dyes and reduced fatigue in triarylmethane photochromic solutions. Purified papain did not bleach the dyes and did not have a significant effect on fatigue in photochromic solutions. It was not as soluble in the alcohol based solvent as the crude enzyme was. It is believed that an impurity is responsible for bleaching triarylmethane dyes and reducing fatigue in triarylmethane photochromic systems.
Library of Congress Subject Headings
Imaging Science (MS)
Austin, Jon H., "The effects of impurities in papain on triarylmethane photochromism" (1983). Thesis. Rochester Institute of Technology. Accessed from
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