Jon H. Austin

Abstract

The effects of the enzyme papain on triarylmethane photochromism were investigated. The interaction between papain solutions and triarylmethane dyes was measured using difference spectroscopy and relative equilibrium constants were calculated. The effects of papain on Malachite Green photochromic solutions were also measured. Both crude and commercially available purified papain were tested. Crude papain bleached triarylmethane dyes and reduced fatigue in triarylmethane photochromic solutions. Purified papain did not bleach the dyes and did not have a significant effect on fatigue in photochromic solutions. It was not as soluble in the alcohol based solvent as the crude enzyme was. It is believed that an impurity is responsible for bleaching triarylmethane dyes and reducing fatigue in triarylmethane photochromic systems.

Library of Congress Subject Headings

Photochromism; Papain

Publication Date

1983

Document Type

Thesis

Student Type

Graduate

Degree Name

Imaging Science (MS)

Advisor

Ronald Francis

Advisor/Committee Member

B. Carroll

Comments

Physical copy available from RIT's Wallace Library at QD715 .A97 1983

Campus

RIT – Main Campus

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