The impedance characteristics of individual cell / electrode systems are used to monitor cellular viability, position, adhesion, and response to external stimuli in hybrid biosensor applications. A planar microelectrode array consisting of 36 platinized iridium electrodes (10 mm diameter) is used as a substrate for the culture of mammalian cells. Electrode impedance is monitored across the array as different environmental factors are changed. Maps of electrode impedance have been shown to correlate directly to cell positioning over an electrode and general cellular viability. Exposure to a well known voltage-gated Na+ channel blocker (tetrodotoxin) provided significant cellular response as compared to control electrodes without cells. The effective use of small electrodes (10 mm diameter) to study single cell / electrode interactions has been demonstrated.

Date of creation, presentation, or exhibit



Solid-State Sensor and Actuator Workshop, Hilton Head Island, South Carolina (1996) This work was funded through a combination of G. Kovacs' NSF NYI Award (No. ECS-9358289) and Terman Fellowship (courtesy of Mr. D. Packard and Mr. W. Hewlett) and a grant from the Office of Naval Research (No. N00014-93-1-0759). D. Borkholder is supported by an Air Force Fellowship.Note: imported from RIT’s Digital Media Library running on DSpace to RIT Scholar Works in February 2014.

Document Type

Conference Proceeding

Department, Program, or Center

Microsystems Engineering (KGCOE)


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