Four wild-type Agrobacterium rhizogenes strains produced morphologically-altered plant structures in three actinorhizal plant species. These structures were transformed tissues as indicated by opine production. Alnus glutinosa (L.) Gaertn. and A. acuminata H.B.K. produced hairy roots containing strain-specific opines from stem wound sites when inoculated with any of the four strains of A. rhizogenes . Alder (Alnus ) hairy roots did not readily proliferate when excised and placed in hormone-free media. Infections by A. rhizogenes strain 8196 gave rise to nodule-like root structures on cotyledons of Russian olive (Elaeagnus angustifolia L.) that failed to proliferate on two hormone-free media. Infections by A. rhizogenes strain K599 on E. angustifolia gave rise to abnormal nodule-like roots, termed pseudoactinorhizae, that continued to proliferate in culture on two hormone-free media. This study demonstrates that A. rhizogenes strains can be used to genetically transform three species of actinorhizal plants, and that in the case of strain K599, infections on E. angustifolia resulted in abnormal nodule-like root tissues which further differentiated into pseudoactinorhizae.

Publication Date



ISSN:1146-609X Note: imported from RIT’s Digital Media Library running on DSpace to RIT Scholar Works in February 2014.

Document Type


Department, Program, or Center

Thomas H. Gosnell School of Life Sciences (COS)


RIT – Main Campus